Anti-CTGF Antibody, N-Term Antibody (C15-1317-748)

General description

Connective tissue growth factor (UniProt P29279; also known as CCN family member 2, Hypertrophic chondrocyte-specific protein 24, IBP-8, IGF-binding protein 8, IGFBP-8, Insulin-like growth factor-binding protein 8) is encoded by the CTGF (also known as CCN2, HCS24, IGFBP8) gene (Gene ID 1490) in human. CTFG is one the three founding members of the CCN (Ctgf, Cyr61, and Nov) family of proteins involved in a wide array of cellular activities, including migration, proliferation, extracellular matrix synthesis, adhesion, survival, differentiation, and apoptosis. CTGF acts an autocrine mediator of transforming growth factor-beta (TGF-beta) in fibroblasts. CCN proteins are characterized by a more than 10% cysteine content and an absolute conservation of the position of their 38 cysteine residues. Human CTGF is synthesized with a signal peptide sequence (a.a. 1-26), the removal of which yields the mature protein with an N-terminal IGF-binding protein (IGFBP) domain (a.a. 27-98), followed by a von Willebrand factor type C (VWC or VWFC) domain (a.a. 101-167), a cysteine-free hinge region (a.a. 168-197), thrombospondin type-1 (TSP-1) domain (a.a. 198-243), and a C-terminal cysteine-knot or C-terminal CYS-KNOT (CTCK) motif (a.a. 256-330). Due to the high number of disulfide bridges, most of the CTGF domains are resistant to proteolytic digestion, and most proteases primarily cleave only in the cysteine-free hinge region, producing almost exclusively intact N- and C-terminal half fragments. The N-terminal half of CTGF is shown to mediate differentiation and collagen synthesis in concert with IGF-2, while its C-terminal half mediates cell proliferation in concert with EGF.

Specificity

This polyclonal antibody was affinity-purified using the purified N-terminal fragment (1-180) from chymotrypsin-cleaved rhCTGF to eliminate immunoreactivity against the C-terminal half (181-349) of CTGF in the original goat antiserum raised against full-length rhCTGF (Grotendorst, G.R., and Duncan, M.R. (2005). FASEB J. 19(7):729-738).

Immunogen

Epitope: N-terminal half.

Recombinant full-length human CTGF.

Application

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

This Anti-CTGF Antibody, N-Term Antibody is validated for use in Western Blotting, Neutralizing for the detection of CTGF.

Western Blotting Analysis: A representative lot detected the N-terminal (1-180), but not C-terminal (181-349), fragment derived from baculovirus expressed human CTGF by chymotrypsin cleavage. The N-terminal fragment appeared as a doublet due to differential glycosylation (Grotendorst, G.R., and Duncan, M.R. (2005). FASEB J. 19(7):729-738).
Neutralizing Analysis: A representative lot inhibited CTGF-stimulated collagen synthesis, but not CTGF-stimulated DNA synthesis, in rat kidney (NRK) fibroblasts (Grotendorst, G.R., and Duncan, M.R. (2005). FASEB J. 19(7):729-738).
Note: It is recommended that gel electrophoresis be performed under non-reducing condition to effectively separate CTGF N- and C-terminal fragments, as well as differentially glycosylated N-terminal fragments.

Quality

Evaluated by Western Blotting of chymotrypsin-cleaved CTGF fragments.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected 1 ng of the N-terminal (1-180), but not C-terminal (181-349), fragment derived from baculovirus expressed human CTGF by chymotrypsin cleavage.

Target description

35.92/38.09 kDa (isoform Long mature/pro-form), 18.95/16.35 kDa (isoform Long N-terminal fragment with/without a.a. 1-26 signal peptide), 33.04/35.21 kDa (isoform Short mature/pro-form) calculated. The N-terminal fragment (a.a. 1-180) generated by chymotrypsin cleavage of glycosylated CTGF can be resolved into a doublet under non-reducing condition with the upper band representing more glycosylated species.

Physical form

Affinity purified.

Purified goat polyclonal antibody in buffer containing 0.3 M Glycine, 50 mM Tris, pH 8.0 without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: goat. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. technique(s): neutralization: suitable, western blot: suitable. NCBI accession no.: NP_001892. UniProt accession no.: P29279. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... CTGF(1490). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.