ANTI-CULLIN-4A/CUL4A CLONE 2A2.1

General description

Cullin-4A (UniProt Q13619; also known as CUL-4A) is encoded by the CUL4A gene (Gene ID 8451) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type (really interesting new gene), HECT-type (homologous to E6-AP C-terminus), and U-box type of E3 ligases. Cullin-4 belongs to the cullin family of hydrophobic proteins that confer substrate specificity to the multimeric Cullin-RING E3 ubiquitin ligase (CRL) complexes by acting as scaffold proteins. The human genome encodes 8 cullin proteins (CUL 1, 2, 3, 4A, 4B, 5, 7 and 9). The activity of CRLs is regulated by Cullin Neddylation via the reversible conjugation of a small ubiquitin-type protein known as Neural precursor cell expressed developmentally down-regulated protein 8 (Nedd8). CUL4A interacts with the substrate-binding adaptor DDB1 (DNA damage binding protein 1) via its arc-shaped helical N-terminal domain, and binds the small RING finger protein ROC1 (regulator of cullins 1) via its globular C-terminal domain. Despite the 83% sequence identity and seemingly functional redundancy between CUL4A and CUL4B, much more focus is placed on CUL4A in oncogenesis research.

Specificity

Clone 2A2.1 is expected to react with both spliced isoforms of human cullin-4A

Immunogen

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to human Cullin-4A/CUL4A near the C-terminus.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-Cullin-4A/CUL4A Antibody, clone 2A2.1 is validated for use in Western Blotting for the detection of Cullin-4A/CUL4A.

Quality

Evaluated by Western Blotting in human melanoma SK-MEL-28 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cullin-4A/CUL4A in 10 µg of human melanoma SK-MEL-28 cell lysate.

Target description

~88 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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