General description
The cell cycle is regulated by the interplay of many molecules. Key among these are the cyclins which are expressed and then degraded in a concerted fashion to drive the stages of the cell cycle. Cyclins combine with cyclin dependent kinases (cdks) to form activated kinases that phosphorylate targets leading to cell cycle regulation. A breakdown in the regulation of this cycle can lead to out of control growth and contribute to tumor formation. Defects in many of the molecules that regulate the cell cycle have been implicated in cancer. Cyclin A is a member of the Cyclin A family that can bind and activate Cdc2 and Cdk2 and is involved in the control of mitosis and S phase. During S phase, cyclin A forms a complex with Cdk2, the pRB-related protein p107 and E2F.
Specificity
p58cyclin A; does not cross-react with cyclin B or cyclin D2, or 3
Immunogen
peptide corresponding amino acid residues 417-432 (C-KHGVSLLNPPETL-N-L)
Application
Detect Cyclin A with Anti-Cyclin A Antibody (Rabbit Polyclonal Antibody), that has been shown to work in IP, WB, ICC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Quality
routinely evaluated by immunoblot on HeLa nuclear extract
Target description
58kDa
Physical form
Format: Purified
Lyophilized
Protein G Chromatography
Storage and Stability
2 years at -20°C
Analysis Note
Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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- UPC:
- 51171809
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 06-138
- Temperature Control Device:
- Yes
