Anti-Cyclin D Antibody (C15-1317-188)

General description

Progression through G1 phase and transition from G1 to S phase of the cell division cycle is controlled by activation of a distinct series of serine/threonine kinase complexes which comprise of a cyclin regulatory subunit and a cyclin dependent kinase (cdk). D type cyclins are induced earlier than cyclin E in G1 and can form complexes with cdk2,-4,-5, and -6. Cyclin D1 is a nuclear protein during G1 phase and disappears from the nucleus in S-phase. Among the key cell cycle regulators, cyclin D1 has been implicated most strongly as a proto-oncogene in several human tumor types, including breast carcinomas.

Specificity

This antibody recognizes Cyclin D at the C-terminus.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to human Cyclin D at the C-terminus.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Use Anti-Cyclin D Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, IP to detect Cyclin D also known as B-cell CLL/lymphoma 1, BCL-1 oncogene.

Western Blot (SNAP ID) Analysis: 5 µg/ml from a previous lot detected Cyclin D on 10 µg of 3T3/A31 cell lysate.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Cyclin D in NIH/3T3, A431, C2C12, and HeLa cells.

Quality

Evaluated by Western Blot in 3T3/A31 cell lysate.

Western Blot Analysis: 1 µg/ml of this antibody detected Cyclin D on 10 µg of 3T3/A31 cell lysate.

Target description

~ 34 kDa

Linkage

Replaces: 04-221

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
3T3/A31 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, mouse. species reactivity (predicted by homology): primate (based on 100% sequence homology). technique(s): immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_444284.1. UniProt accession no.: P24385. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... CCND1(595). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.