Anti-Cysteine String Protein Antibody (C15-1315-825)

General description

Cysteine-string proteins are membrane-bound proteins associated primarily with nerve endings and synaptic vesicles in the brain where they serve an important role in presynaptic function and may be involved in calcium-dependent neurotransmitter release at nerve endings. CSPs posses a strech of amino acids highly enriched in cysteine residues that are post-translationally palmitoylated. CSPs may function as homodimers.

Specificity

Cysteine-string Protein. The antibody recognizes primarily the alpha isoform of CSP with some recognition of the beta isoforms. Slight reactivity with the gamma isoforms may be detected.

Immunogen

Recombinant rat cysteine-string protein with the cysteine string deleted.

Application

Immunohistochemistry on formalin fixed tissue: 1:1,000. (Recommend Cat. No. AB1576P).Immunocytochemistry on PC-12 cells: 1:1,000. (Recommend Cat. No. AB1576P).Immunoblotting: 1:5,000. CSPs should be resolved on 12-15% SDS-PAGE gels under NON-REDUCING conditions. CSPs typically migrate as 33-35 kDa species in vertebrate samples (dimers may also be seen). If one must perform SDS-PAGE under reducing conditions, it is necessary to alkylate the CSPs to prevent smearing.To ensure proper identification of CSPs, samples may be deacylated by treatment with 1M neutral hydroxylamine or 0.1M KOH in methanol resulting in a 6-7 kDa shift in electrophoretic mobility of the CSP band. Deacylation will usually result imn some loss of reactivity on western blots so sufficient protein should be loaded. Alternatively, because all CSPs appear to be acidic (approximate pI of 4.6-4.8) 2-D gel electrophoresis may be usefull to distinguish some CSPs. Finally, some sample treatments may cause partial deacylation of CSPs resulting in a smear of immunoreactivity below the mass of the fatty acylated monomer.Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Synapse & Synaptic Biology

This Anti-Cysteine String Protein Antibody is validated for use in WB, IC, IH for the detection of Cysteine String Protein.

Physical form

Liquid rabbit serum containing no preservatives

Storage and Stability

Store at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
POSITIVE CONTROL: brain samples

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity (predicted by homology): all. manufacturer/tradename: Chemicon^®. . technique(s): western blot: suitable. NCBI accession no.: NM_025219.1. UniProt accession no.: Q9H3Z4. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... DNAJC5(80331). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.