Specificity
The rabbit polyclonal serum detects CYP3A4 in humans and rats; by sequence homology of the immunogen used, cross reactivity to CYP3A7, 3A6v4;3A2v1, 3A8, 3A9 may be expected but is untested at this time.
Immunogen
A synthetic peptide containing the C-terminal sequence of TVSGA from human CYP3A4. The peptide was coupled to KLH.
Application
Anti-Cytochrome P450 Enzyme CYP3A4 Antibody detects level of Cytochrome P450 Enzyme CYP3A4 & has been published & validated for use in IH & WB.
Research Category
Metabolism
Research Sub Category
Enzymes & Biochemistry
Western blot: 1:1000-1:2000: Using microsomal membrane fractions, detects a band at ~54kDa; other bands may be present as well. Antibody has been successfully used to detect CYP3A4 on LICOR fluorescent westerns as well.
Immunohistochemistry: 10% formalin fixed, paraffin embedded tissues 1:200-1:400, microwave-citric acid antigen recovery is suggested.
Optimal working dilutions must be determined by end user.
Target description
57 kDa
Physical form
Rabbit antiSerum. Liquid containing no preservative.
Unpurified
Storage and Stability
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Liver tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human, pig. species reactivity (predicted by homology): mammals, rat. manufacturer/tradename: Chemicon®. . technique(s): immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NM_017460.3. UniProt accession no.: P08684. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... CYP3A4(1576). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 41116127
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB1254
