General description
The cytokeratins are a family of intermediate filaments found in almost all epithelial cells. They are divided into four subclasses depending on their patterns of reactivity and electrophoretic characteristics and are also arranged I a numbered series of 20 or more acoring to molecular weight and isoelectric point (Quinlan et al. 1985). Types 5 and 6 of cytokeratin (CK5/6) are in high frequency in mesothelial cells, as opposed to cells of adenocarcinoma. CK5/6 has been shown to be a reliable marker for mesothelioma and squamous cell carcinoma of the lung and Bowen′s disease. It does not react with pulmonary adenocarcinoma.
Specificity
Expected to cross-react with mouse and rat.
Reacts with human cytokeratin 5 on immunoblots of cytoskeletal preparations of epidermis and non-cytokeratinizing epithelium (Abstract: European Symposium of the Biology of the Cytoskeleton, Helsinki, 18-21, 6, 89, 1989) and on tissue sections. Shows reactivity with cytokeratin 6 and weak reactivity with cytokeratin 4 on immunoblots. No cross-reactivity with cytokeratins 1, 7, 8, 10, 13, 14, 18 or 19. MAB1620 recognizes basal cells and a part of the stratum spinosum in the normal pavement epithelium. The antibody may be useful for distinction of low differentiated pavement epithelium carcinoma and adenocarcinoma. The antibody works on formalin fixed tissues.
Immunogen
Purified cytokeratin 5 (Exp. Cell Res. (1986) 162:114; J. Invest. Dermatology (1987) 88:191).
Application
Anti-Cytokeratin 5 Antibody, 6, clone D5/16B4 is an antibody against Cytokeratin 5 for use in IF, IH(P) & WB.
Immunohistochemistry (frozen or paraffin sections):
A 1:50-1:100 dilution from a previous lot was used for paraffin, high-temperature antigen retrieval required (Pressure cooker, Citrate/EDTA buffer pH 6.0, 4 minutes at full pressure).
Immunofluorescence:
A previous lot of this antibody was used in Immunofluorescence.
Western blot:
Recognizes a 58kDa and 56kDa band corresponding to cytokeratin 5 and 6.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
Quality
Routinely evaluated by Western Blot on A431 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected cytokeratin 5/6 on 10 ug of A431 lysates.
Target description
Cytokeratin 4 (59 kDa), Cytokeratin 5 (58 kDa) and Cytokeratin 6 (56 kDa).
Physical form
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
Storage and Stability
Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
Analysis Note
Control
A431 cell lysate, human bladder tumor tissue.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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- UPC:
- 41116015
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MAB1620
- Temperature Control Device:
- Yes
