ANTI-DAG KINASE ALPHA CLONE 10D6.1 (C005B-450944)

General description

Diacylglycerol kinase alpha (UniProt: P23743; also known as EC:2.7.1.107, DAG kinase alpha, 80 kDa diacylglycerol kinase, Diglyceride kinase alpha, DGK-alpha) is encoded by the DGKA (also known as DAGK, DAGK1) gene (Gene ID: 1606) in human. Diacylglycerol kinase is a lipid kinase that converts diacylglycerol to phosphatidic acid, and regulates the activity of many enzymes including protein kinase C, phosphatidylinositol 4-phosphate 5-kinase, and mTOR. Thus far ten mammalian subtypes of DAG kinase have been reported. All DAG kinase with the exception of beta isoform are localized in cytoplasm in several cells, but some translocate to the plasma membrane upon activation. DAG kinase acts as a modulator, which competes with protein kinase C for diacylglycerol in intracellular signaling pathways. DAG kinase also plays a role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Most DAG kinases, with the exception of theta, contain two cysteine-rich regions (C1A and C1B domains) in the regulatory domain of the N-terminal half of the molecule. In DAK kinase alpha the C1 domain closest to the catalytic domain has an extended region of fifteen amino acids that is not present in C1 domains of other DAG kinases. DAG kinase alpha can be activated by the phosphorylation of Tyr335, which occurs during T cell activation or following exposure of cells to hepatocyte growth factor.

Specificity

Clone 10D6.1 detects DAG kinase alpha in human. It targets an epitope within the first 110 amino acids from the N-terminal region.

Immunogen

GST/His-tagged recombinant fragment corresponding to the first 110 amino acids of human DAG kinase alpha.

Application

Anti-DAG Kinase Alpha, clone 10D6.1, Cat. No. MABF1105, is a mouse monoclonal antibody that detects DAG kinase alpha. It has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected DAG Kinase Alpha in human tonsil and human breast cancer tissue.

Research Category
Inflammation & Immunology

Quality

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected DAG Kinase Alpha in 10 µg of Jurkat cell lysate.

Target description

~83 kDa observed; 82.63 kDa calculaed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.