ANTI-DAP KINASE 1 CLONE 1J22 ZOOMAB RABBIT MONOCLONAL (C15-1670-656)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1J22 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Death-associated protein kinase 1 (DAP Kinase 1). It targets an epitope within 18 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of human Death-associated protein kinase 1 (DAP Kinase 1).

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected DAP Kinase1 in HeLa cell lysate.

Tested applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected DAP Kinase1 in mouse and rat liver tissue lysates.

Affinity Binding Assay: A representative lot of this antibody bound DAP Kinase 1 with a KD of 8.8 x 10-11 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected DAP Kinase1 in human placenta tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected DAP Kinase1 in HeLa, A431, and NIH3T3 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-DAP Kinase 1, clone 1J22 ZooMAb^®, Cat. No. ZRB1402, is a recombinant Rabbit monoclonal antibody that targets DAP kinase 1 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Target description

Death-associated protein kinase 1 (UniProt: P53355; also known as EC:2.7.11.1, DAP kinase 1) is encoded by the DAPK1 (also known as DAPK) gene (Gene ID: 1612) in human. DAP Kinase 1 is a calcium/calmodulin-dependent serine/threonine kinase that is involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. It regulates both type I, caspase-dependent apoptotic, and type II, caspase-independent, autophagic cell deaths signal, depending on the cellular setting. The latter is characterized by the accumulation of autophagic vesicles. It is reported to phosphorylate PIN1, which results in inhibition of catalytic activity, nuclear localization, and cellular function. It is also shown to phosphorylate protein kinase D1 (PRKD1) and regulate JNK signaling by binding and activating PRKD1 under oxidative stress. It can phosphorylate Beclin-1 and reduce its interaction with Bcl-2 and Bcl-2L1 and promote the induction of autophagy. In response to mitogenic stimuli, such as phorbol ester (PMA) and EGF, DAP kinase 1 undergoes phosphorylation at serine 289 that suppresses its pro-apoptotic function. It is also reported to be phosphorylated at serine 734 by MAP kinase 1, which increases its catalytic activity. However, autophosphorylation at serine 308 inhibits its catalytic activity. DAP kinase 1 activity is regulated by a locking mechanism, involving autophosphorylation at serine 308 and calmodulin binding. In the inactive state, serine 308 is phosphorylated and its activation involves its dephosphorylation and a release-of-autoinhibition mechanism. Binding of calmodulin induces a conformational change that relieves the steric block of the active site by the autoinhibitory domain. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb^® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.