General description
Natural killer (NK) cells are lymphocytes that function as a component of innate immunity against intracellular and parasitic pathogens, and possibly tumors. DAP12 is expressed on the surface of NK cells as a disulfide-linked homodimer and contains an immunoreceptor tyrosine based activation motif (ITAM) in its cytoplasmic domain. DAP12 non-covalently associates with members of the killer cell immunoglobulin-like receptor (KIR) family resulting in cellular activation.
Specificity
Recognizes human DAP12. This antibody detects a band of approximately 12 kDa in Western blot analysis of U937 cell lysate.
Immunogen
Synthetic peptide corresponding to amino acid residues 89-113 of human DAP12 (Accession # AAD09437).
Application
Anti-DAP12 Antibody is an antibody against DAP12 for use in WB.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Western blot: 1.0 μg/mL
Optimal working dilutions must be determined by the end user.
Physical form
Format: Purified
Protein A purified immunoglobulin. Liquid in 0.02M Phosphate Buffer, 0.25M NaCl, with 0.1% sodium azide.
Storage and Stability
Maintain at 2-8°C for up to 12 months from date of receipt.
Analysis Note
Control
Western blot positive control: U937 cell line
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human. manufacturer/tradename: Chemicon®. . technique(s): western blot: suitable. NCBI accession no.: NM_003332.2, NM_198125.1. UniProt accession no.: O43914. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... TYROBP(7305). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51202407
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB3890
