General description
DAP12 is a transmembrane adaptor protein that is non-covalently associated with several cell surface receptors on natural killer (NK), myeloid, and presumably neuronal cells (Bakker, 2000, Campbell, 1999). DAP12 contains an ITAM domain that preferentially recruits the protein tyrosine kinase Syk to initiate signal transduction (McVicar, 1998). Deficiency of DAP12 results in reduced antigen presentation function by myeloid cells and defects in function of certain NK cell receptors in mice (Bakker. 2000, Tomasello, 2000), as well as presenile dementia and bone cysts in humans (Paloneva, 2000).
Specificity
Recognizes DAP12 protein.
Immunogen
Recombinant DAP12 protein.
Application
Anti-DAP12 Antibody detects level of DAP12 & has been published & validated for use in IP.
Immunoprecipitation (2-4 μg/mg of protein lysate)
Optimal working dilutions must be determined by end user.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Physical form
Format: Purified
Purified immunoglobulin. Liquid in phosphate buffered saline with 0.08% sodium azide.
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
Analysis Note
Control
POSITIVE CONTROL: NKp44 positive cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. species reactivity: mouse, human. manufacturer/tradename: Chemicon®. . technique(s): immunoprecipitation (IP): suitable. NCBI accession no.: NM_003332.2, NM_198125.1. UniProt accession no.: O43914. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... TYROBP(7305). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51202407
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB4070
