Specificity
Recognizes DARPP-32 phosphorylated at threonine 34 .
Immunogen
Epitope: phosphoThr34
Synthetic peptide from DARPP-32, phosphoThr34.
Application
Research Category
Neuroscience
Research Sub Category
Reward & Addiction
Signaling Neuroscience
This Anti-DARPP-32 Antibody, phosphoThr34 is validated for use in WB for the detection of DARPP-32.
Western blot: 1:1,000. The antibody recognizes the ~32 kD DARPP-32 protein phosphorylated at Thr34 protein in rat caudate lysate prepared from caudate slices that had been incubated in the absence and presence of forskolin. The labeling by the antibody to DARPP-32 Thr34 is markedly increased by the forskolin treatment.
Optimal working dilutions must be determined by the end user.
Target description
~32 kD
Physical form
Affinity Purified immunoglobulin. Liquid in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg per mL BSA with 50% glycerol.
ImmunoAffinity Purified
Storage and Stability
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Cultured primary neurons, rat brain lysate and human breast adenocarcinoma
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, mouse, rat. manufacturer/tradename: Chemicon®. . technique(s): western blot: suitable. NCBI accession no.: NM_032192.2, NM_181505.2. UniProt accession no.: Q9UD71. shipped in: dry ice. target post-translational modification: phosphorylation (pThr34). Gene Information: human ... PPP1R1B(84152). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2.- UPC:
- 51202416
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB9206
