Anti-Dax1 Antibody (C15-1316-831)

General description

Dax-1 is an orphan nuclear receptor and is required for the normal development of the hypothalamic-pituitary-adrenal-gonadal axis formation. Defects in Dax-1 are the cause of X-linked adrenal hypoplasia congenital (XL-AHC); also known as X-linked Addison disease (AHX). Dax-1 also has a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency by acting as a negative regulator of Oct3/4 in ES cells.

Specificity

75% Homologogous to Mouse Dax1

Immunogen

KLH-conjugated linear peptide corresponding to the Region of Ligand binding of Human Dax1.

Application

Evaluated by Immunohistochemistry in Human and Rat adrenal gland tissue. Immunohistochemsitry Analysis: A 1:1000 dilution of this antibody detected Dax1 in Human and Rat glioblastoma adrenal gland tissue.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

This Dax1 antibody is validated for use in WB & IHC for the detection of the Dax1 protein.

Quality

Evaluated by Western Blot in a Dax1 overexpressing HEK293 transfected cell line. Western Blot Analysis: A 1:2000 dilution of this antibody detected Dax1 in a Dax1 overexpressing HEK293 transfected cell line.

Target description

~53 kDa observed

Linkage

Replaces: 04-445

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Dax1 overexpressing HEK293 transfected cell line

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat, human. technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable. NCBI accession no.: NP_000466. UniProt accession no.: P51843. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... NR0B1(190). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.