ANTI-DAXX CLONE DAXX5.14 (C15-1887-749)

General description

Cytoplasmic polyadenylation element binding protein 4 (UniProt: D3ZKL3; also known as CPEB4) is encoded by the Cpeb4 (also known as rCG_33472) gene (Gene ID: 303010) in rat. The CPEB family of RNA-binding proteins regulate translation in various tissues in vertebrates. They shuttle between nucleocytoplasmic compartments with longer retention in the cytoplasm where they regulate target RNA translation. CPEB4 is an RNA-binding protein that promotes polyadenylation and translation of target RNAs and contributes to meiotic and mitotic progression. It acts as a survival factor exclusively for early postnatal granule cells in the olfactory bulb. CPEB4 is reported to interact with adaptor protein vinexin by binding to its first two SH3 domains and this interaction leads to vinexin localization in stress granules. It is shown that CPEB4 recruits vinexin to stress granules in response to adverse conditions, such as heat shock or arsenite-induced stress. CPEB4 is also shown to be essential for cell survival and becomes nuclear in response to focal ischemia in vivo and when cultured neurons are deprived of oxygen and glucose. (Ref.: Tseng, CS., et al. (2017). Cell Reports 21, 2264-2276; Chang, YW., and Huang, YS. (2014). PLoS ONE 9(9); e107961; Kan, MC., et al. (2010). Mol. Cell Biol. 30(24); 5658-5671).

Specificity

Clone A4 is a mouse monoclonal antibody that detects Cytoplasmic polyadenylation element binding protein 4 (CPEB4). It targets an epitope within the N-terminal region.

Immunogen

Recombinant fragment corresponding to the first 458 amino acids from the N-terminal region of rat CPEB4.

Application

Quality Control Testing

Evaluated by Western Blotting in Neuro-2A cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CPEB4 in Neuro-2A cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CPEB4 in A375 and HepG2 cell lysates.

Immunoprecipitation Analysis: A representative lot immunoprecipitated CPEB4 in Immunoprecipitation applications (Chang, Y.W., et al. (2014). PLoS One. 9(9):e107961).

Immunocytochemistry Analysis: A representative lot detected CPEB4 in Immunocytochemistry applications (Chang, Y.W., et al. (2014). PLoS One. 9(9):e107961).

Western Blotting Analysis: A representative lot detected CPEB4 in Western Blotting applications (Chang, Y.W., et al. (2014). PLoS One. 9(9):e107961; Tseng, C-S., et al. (2017). Cell Rep. 21(8):2264-2276).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-CPEB4, clone A4, Cat. No. MABE1915, is a mouse monoclonal antibody that detects CPEB4 and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.