General description
DDX4 (Vasa or MVH) is a member of the DEAD family of proteins and is a probable ATP-dependent RNA helicase. DDX4 (Vasa) proteins are mostly localized in testicular germ cells in the cytoplasm of spermatocytes. DDX4 (Vasa) is a high-copy extragenic suppressor gene. DDX4 (Vasa) is shown to be significantly decreased in spermatozoa of men that are infertile and may have an important role in male fertility.
Specificity
This antibody recognizes DDX4 (Vasa) at the C-terminus.
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of mouse DDX4 (Vasa).
Application
Anti-DDX4 (Vasa) Antibody detects level of DDX4 (Vasa) & has been published & validated for use in WB, IH(P).
Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected DDX4 (Vasa) in normal human testis tissue.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
RNA Metabolism & Binding Proteins
Quality
Evaluated by Western Blot in mouse testis tissue lysate.
Western Blot Analysis: A 1:500 dilution of this antibody detected DDX4 (Vasa) in 10 µg of mouse testis tissue lysate. Western Blot performed without Tween.
Target description
~76 kDa observed
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse testis tissue lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat, human, mouse. technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable. NCBI accession no.: NP_034159. UniProt accession no.: Q61496. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... DDX4(54514). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51202407
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB4330