Anti-DEDAF Antibody (C15-1316-117)

Specificity

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. DEDAF (DED Associated Factor) is expressed in multiple tissues and cell lines. DEDAF is identical to the transcriptional repressor RYBP. DEDAF interacts with FADD and augments the formation of CD95/FADD/Caspase-8 complexes at the cell membrane. DEDAF also interacts with DEDD (DED-containing DNA binding) protein in the nucleus, which indicates its involvement in the regulation of both cytoplasmic and nuclear events of apoptosis.

Immunogen

Rabbit anti-DEDAF polyclonal antibody was raised against a synthetic peptide corresponding to amino acids 215 to 228 of human-DEDAF. This sequence is identical to the mouse sequence.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-DEDAF Antibody is validated for use in WB for the detection of DEDAF.

Western blot: 0.5-1 μg/mL.

A549 or HepG2 cell lysate can be used as a positive control and a 32 kDa band should be detected.

Optimal working dilutions must be determined by end user.

Physical form

Format: Purified

Purified IgG by immunoaffinity chromatography. Liquid in PBS containing 0.02% sodium azide.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human, rat, mouse. manufacturer/tradename: Chemicon^®. . technique(s): western blot: suitable. NCBI accession no.: NM_012234.4. UniProt accession no.: Q8N488. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... RYBP(23429). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.