Anti-DEPTOR Antibody (C15-1317-842)

General description

DEPTOR (DEPDC6, DEP domain-containing protein 6) was recently identified as a novel mTOR-interacting protein whose over-expression in certain cell types represents a new mechanism for activating PI3K/Akt signaling and promoting cell survival. DEPTOR negatively regulates mTORC1 and mTORC2. It has been shown that loss of DEPTOR activates various targets that include p70S6K1, Akt, and SGK1, that leads to cell growth and survival. Little was known about DEPDC6 prior to the discovery of its interactions with mTOR, at which time it was given the new name of DEPTOR (Peterson, T.R., et al. (2009). Cell. 137(5):873-876).

Specificity

Other homologies: Primate, Equine, Porcine, Canine, Rat, and Mouse based on 99%, 99%, 98%, 97%, and 96% homology, respectively.

This antibody recognizes the DEP domain that contains mTOR-interacting protein DEPTOR.

Immunogen

Epitope: DEP domain-containing mTOR-interacting protein DEPTOR.

KLH-conjugated linear peptide corresponding to the DEP domain-containing mTOR-interacting protein of human DEPTOR.

Application

Anti-DEPTOR Antibody detects level of DEPTOR & has been published & validated for use in WB.

Research Category
Metabolism

Research Sub Category
PI3K, Akt, & mTOR Signaling

Quality

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected DEPTOR in HEK293 cell lysate.

Target description

~46 kDa observed.

Linkage

Replaces: 09-463

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HEK293 cell lyaste

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. technique(s): western blot: suitable. NCBI accession no.: NP_073620. UniProt accession no.: Q8TB45. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... DEPTOR(64798). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.