ANTI-DEXRAS1 CLONE 4B11.1

General description

Dexamethasone-induced Ras-related protein 1 (UniProt Q9Y272; also known as Activator of G-protein signaling 1) is encoded by the RASD1 (also known as AGS1, DEXRAS1) gene (Gene ID 51655) in human. Rasd1/Dexras belongs to the monomeric Ras superfamily of GTPases that are commonly referred to as small G-proteins. Rasd1/Dexras1 is a small G protein that functions as an important signal transducer in regulating circadian rhythms, GH secretion, and cell growth. Rasd1/Dexras is rapidly up-regulated by the synthetic glucocorticoid dexamethasone as a result of GR-STAT5b complex-mediated transcription. Rasd1/Dexras1 interacts with neuronal nitric oxide synthase (nNOS or bNOS) via the scaffolding protein CAPON, with nitric oxide serving as a guanine nucleotide exchange factor for Rasd1/Dexras1. Rasd1/Dexras1 is induced by glucocorticoids during adipogenic differentiation. Exogenous Rasd1/Dexras1 expression rescues impaired adipogenesis of Dexras1-deficient mouse embryonic fibroblasts (MEFs). Dexras1 knockout mice display impaired adiposity and are resistant to diet-induced weight gain. Human, moue, and rat Rasd1/Dexras1 protein is initially produced with a 3-amino acid propeptide sequence at the C-terminal end, which was posttranslationally removed to yield the mature protein.

Specificity

Clone 4B11.1 detects an epitope conserved among human, mouse, and rat RASD1 protein, but missing in human RASD1 spliced isoform 2 reported by UniProt (Q9Y272).

Immunogen

Epitope: Internal.

GST-tagged recombinant human DEXRAS1 internal fragment.

Application

Anti-DEXRAS1 Antibody, clone 4B11.1 is an antibody against DEXRAS1 for use in Western Blotting, Immunohistochemistry (Paraffin).

Research Category
Neuroscience

Research Sub Category
G-proteins

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected DEXRAS1 in 10 µg of mouse brain tissue lysate.
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected DEXRAS1 in rat testis, mouse cerebellum, and human kidney tissue sections.

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected DEXRAS1 in 10 µg of rat brain tissue lysate.

Target description

~32 kDa oberved. 31.64/31.68/31.71 kDa (human/mouse/rat pro-form) and 31.34/31.38/31.41 kDa (human/mouse/rat mature form) calculated.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.