Anti-DGKH antibody produced in rabbit (C15-1456-154)

General description

Diacylglycerol kinase η (DGKH) is encoded by the gene mapped to human chromosome 13q14. The encoded protein belongs to the type II diacylglycerol kinase (DGK) enzyme family. DGKH has two alternatively spliced transcript variants encoding different isoforms namely, η1 and η2. Both the isoforms contain catalytic a and b subdomains and N-terminal pleckstrin homology domain (PH) in common. DGKH η2 isoform has sterile α-motif domain at its C terminal end, whereas it is absent in η1 isoform.

Immunogen

diacylglycerol kinase, eta recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Biochem/physiol Actions

Diacylglycerol kinase η (DGKH) is a vital constituent of lithium-sensitive phosphatidyl inositol pathway. The encoded protein plays an essential role in the regulation of intracellular concentrations of two bioactive lipids, including diacylglycerol and phosphatidic acid. DGKH functions as a potential cellular sensor for phosphatidylinositol (PI) 4,5-bisphosphate (PI(4,5)P2). Mutations in the gene are associated with bipolar disorder (BPD), adult attention-deficit/hyperactivity disorder (aADHD) and unipolar depression (UPD). In addition, variations in the gene expression also increase the risk of susceptibility to calcium oxalate stone and hypercalciuria.

Features and Benefits

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST81294.

Physical form

Solution in phosphate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide.

Legal Information

Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.