General description
Anti-Digoxigenin-Fluorescein, Fab fragments has fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to fluorescein.
Specificity
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.
Application
Anti-Digoxigenin-Fluorescein, Fab fragments are used for the detection of digoxigenin-labeled compounds using:
- Digoxigenin-labeled sugars in glycoconjugate research
- Fluorescent in situ hybridization (FISH)
- Immunohistocytochemistry
- In situ hybridization
Preparation Note
- Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:Detection of digoxigenin-labeled sugars in glycoproteins: 50 to 20μg/ml
- Fluorescent in situ hybridization (FISH): 1 to 20μg/ml
- Immunohistocytochemistry: 20 to 50μg/ml
- In situ hybridization: 20 to 50μg/ml
The anti-DIG anitbody is highly specific and sensitive. Therefore, for FISH, a concentration of 1μg/ml is sufficient, and only in case of critical probes, this concentration may be raised to <10μg/ml (with a distinct background to be expected).
Working solution: Reconstitution
Add 1 ml double-distilled water to a final concentration of 200μg/ml.
Blocking:
PBS, 0.5% bovine serum albumin (w/v), pH 7.4.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding. Furthermore, pH can be increased up to pH 8.5 to 9.0.
Storage conditions (working solution): Always prepare fresh!
After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.
Reconstitution
Add 1 ml double-distilled water to a final concentration of 200 μg/ml.
Other Notes
For life science research only. Not for use in diagnostic procedures.
- UPC:
- 51282130
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 11207741910
