Anti-dimethyl Histone H3 (Arg26), Asymmetric, Trial Size Antibody (C15-1317-147)

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specificity

Broad species reactivity is expected based on immunogen sequence similarities.

Immunogen

KLH-conjugated linear peptide corresponding to a region near the N-terminus of human Histone H3, asymmetrically dimethylated at Arg26.

Application

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected dimethyl Histone H3 (Arg26), Asymmetric in A431 and HepG2 cells.

Dot Blot (Specificity) Analysis: 0.5 µg/mL from a representative lot detected dimethyl Histone H3 (Arg26), Asymmetric in Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).

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Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

This Anti-dimethyl Histone H3 (Arg26) antibody, Asymmetric, Trial Size is validated for use in western blotting, ICC & dot blot for the detection of dimethyl Histone H3 (Arg26).

Quality

Evaluated by Western Blotting in untreated and CARM1 treated HeLa acid extracts.

Western Blotting Analysis: 1 µg/mL of this antibody detects dimethyl Histone H3 (Arg26), Asymmetric in 10 µg of CARM1 treated HeLa acid extract.

Target description

~17 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. technique(s): dot blot: suitable, immunocytochemistry: suitable, western blot: suitable. NCBI accession no.: NP_003520. UniProt accession no.: P68431. shipped in: wet ice. target post-translational modification: dimethylation (Arg26). Gene Information: human ... HIST1H3F(8968). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.