Anti-DISC1 Antibody (C15-1317-540)

General description

Disrupted in schizophrenia 1 (DISC1) is a candidate gene for susceptibility to schizophrenia. It was discovered through chromosomal analysis of a large Scottish family whose members exhibited schizophrenia and related psychiatric disorders. Through yeast two-hybrid screening, it was discovered that DISC1 interacts with many members of the centrosome and cytoskeletal system including MAP1A and Nudel. More recently, DISC1 has been found to regulate the transport of a complex containing Nudel, the lissencephaly-1 (LIS1) protein, and 14-3-3epsilon from neuronal cell bodies to the axons by the action of the microtubule-dependent directed motor protein kinesin-1, also known as KIF5A. Decreased expression of DISC1 in neurons caused an accelerated rate of neuronal integration, resulting in aberrant morphological development, suggesting that DISC1 plays a role in dendritic development and synapse formation. DISC1 has at least four known isoforms.

Immunogen

KLH-conjugated linear peptide corresponding to human DISC1.

Application

Anti-DISC1 detects levels of DISC1 proteins & has been published & validated for use in IHC, ICC & WB.

Immunohistochemistry Analysis: A 1:2,000 dilution of this antibody lot detected DISC1 in rat cerebellum tissue.

Immunocytochemistry Analysis: A 1:2,000 dilution from a representative lot detected DISC1 in HeLa cells.

Western Blot Analysis: 2 µg/mL from a representative lot detected DISC1 in 10 µg/mL of SK-N-SH cell lysate and mouse brain tissue lysate.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Immunohistochemistry in human brain tissue.

Immunohistochemistry Analysis: A 1:2,000 dilution of this antibody lot detected DISC1 in human brain tissue.

Target description

~94 kDa observed. Uniprot describes 8 isoforms ranging between ~39 kDa and ~94 kDa; however, there are many additional isoforms of DISC1 known to exist. (Ishizuka, K., et al. (2007). Molecular Psychiatry. 12:897-899.)

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat, mouse, human. technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable. UniProt accession no.: Q9NRI5. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... DISC1(27185). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.