Anti-DLK1 Antibody (C15-1317-569)

General description

Delta-like 1 homolog (DLK1), also known as fetal antigen 1 (FA1) and preadipocyte factor 1 (pref-1), is a transmembrane protein containing 6 epidermal growth factor repeats whose expression is known to modulate differentiation signals. Studies have shown DLK1 is involved in differentiation of several cell types, including adipocytes. Studies have shown deficiencies in DLK1 displaying growth retardation, obesity, skeletal malformation, and increased serum lipid. DLK1 is a paternally expressed imprinted gene that plays a role in normal development and in the maintence of homeostatsis of adipose tissue mass.

Immunogen

KLH-conjugated linear peptide corresponding to human DLK1.

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected DLK1 in mouse embryonic tissue.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

This Anti-DLK1 Antibody is validated for use in WB, IH for the detection of DLK1.

Quality

Evaluated by Western Blot in HepG2 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected DLK1 on 10 µg of HepG2 cell lysate.

Target description

~47 kDa observed. The calculated molecular weight of this protein is 41 kDa, but can be observed at ~41-55 kDa due to high glycosylation.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HepG2 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat, human, mouse. technique(s): immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NP_003827. UniProt accession no.: P80370. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... DLK1(8788). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.