Anti-Dog IgG (whole molecule)-Peroxidase antibody produced in rabbit (C15-1315-372)

General description

IgG (immunoglobulin G) antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. Mammalian IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c.

Specificity of the anti-dog IgG antiserum is determined by immunoelectrophoresis, prior to conjugation, versus normal dog serum and dog IgG.

Application

Anti-Dog IgG (whole molecule) Peroxidase antibody produced in rabbit has been used:

• for the detection of antibodies against noro viruses, by ELISA (enzyme linked immunosorbent asay) at 1:2000 for 1 hour at 37°C
• in IgG (Immunoglobulin G) staining method
• in ELISA to detect the incorporation of RVGTM (a rabies virus G protein variant)

Biochem/physiol Actions

IgG (Immunoglobulin G) antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. Anti-Dog IgG antibody is a secondary antibody. Second antibodies (secondary antibodies) are raised against primary antibodies, which bind to specific antigens to create antigen antibody complexes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 0.05% MIT

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in "Immunofluorescence and Related Staining Techniques," Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Legal Information

Ampliflu is a trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 200. conjugate: peroxidase conjugate. antibody form: affinity isolated antibody. antibody product type: secondary antibodies. clone: polyclonal. form: buffered aqueous solution. technique(s): direct ELISA: 1:10,000. shipped in: dry ice. storage temp.: −. 20°C. target post-translational modification: unmodified. Pictograms: GHS07. Signal Word: Warning. Hazard Statements: H317. Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364. Hazard Classifications: Skin Sens. 1. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.