ANTI-DOM3Z (C15-1316-868)

General description

Decapping and exoribonuclease protein (DOM3Z), also known as DXO, Dom-3 homolog Z, and encoded by the gene symbol DOM3Z/ DOM3L/ NG6, is a ribonuclease that specifically degrades pre-mRNAs with a defective 5′ end cap. DOM3Z is part of a pre-mRNA capping quality control and has decapping, pyrophosphohydrolase and 5′-3′ exonuclease activities. DOM3Z has a decapping activity toward incomplete 5′ end cap mRNAs (such as unmethylated 5′ end-capped RNA), which in return releases GpppN and 5′ end monophosphate RNA. The 5′ end monophosphate RNA is then degraded by the 5′-3′ exoribonuclease activity, enabling this enzyme to decap and degrade incompletely capped mRNAs. Additionally, DOM3Z has been implied to have RNA 5′-pyrophosphohydrolase activity and to release pyrophosphates by hydrolyzing the 5′ end triphosphate. DOM3Z is located in the nucleus, and expressed ubiquitously.

Immunogen

KLH-conjugated linear peptide corresponding to human DOM3Z.

Application

Anti-DOM3Z is an antibody against DOM3Z for use in Western Blotting.

Quality

Evaluated by Western Blotting in human pancreas tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected DOM3Z in 10 µg of human pancreas tissue lysate.

Target description

~45 kDa observed

Other Notes

Concentration: Please refer to lot specific datasheet.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse, rat, human. species reactivity (predicted by homology): bovine (based on 100% sequence homology). technique(s): western blot: suitable. NCBI accession no.: NP_005501. UniProt accession no.: O77932. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... DXO(1797). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.