ANTI-DOPAMINE D4 RECEPTOR RABBIT PAB (C15-1302-773)

General description

Anti-Dopamine D4 Receptor, rabbit polyclonal, recognizes ~48-51 kDa dopamine D4 receptor in rat brain. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB & IHC.

Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~48-51 kDa D₄ dopamine receptor.

Recognizes the ~48-51 kDa dopamine D₄ receptor in rat brain. Does not cross-react with other dopamine receptors.

Immunogen

Human

a synthetic peptide corresponding to amino acids 176-185 of human dopamine D₄ receptor

Application

ELISA (see comments)

Immunoblotting (1:500)

Immunohistochemistry (1:10,000)

Warning

Toxicity: Standard Handling (A)

Physical form

Undiluted serum.

Reconstitution

Reconstitute the lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antiserum use a similar buffer formulation. Following reconstitution, refrigerate (4°C). Variables associated with assay conditions will dictate the proper working dilution.

Analysis Note

Positive Control
Rat brain

Other Notes

Mignini, F., et al. 2000. J. Autonom. Pharmacol.20, 325.

This antibody has also been reported to work for ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat′s milk. For immunohistochemistry, a 1:10,000 dilution of the reconstituted serum demonstrated specific staining of rat brain sections including olfactory bulb glomeruli, cortex layer 4, cortex layers 2 and 3 (dendrite staining), cortex layer 5 (apical dendrite staining), and the dentate gyrus of the hippocampus (diffuse staining in the molecular layer, punctuate staining in the granule cell layer).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. form: lyophilized. does not contain: preservative. species reactivity: human, rat. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze. isotype: IgG. shipped in: ambient. storage temp.: 2-8°C. target post-translational modification: unmodified. Gene Information: human ... DRD4(1815)
rat ... Drd4(25432). Storage Class Code: 11 - Combustible Solids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.