ANTI-DOPAMINE D2 RECEPTOR ANTIBODY CYTOPLASMIC DOMAIN LONG FORM (C15-1315-894)

Specificity

Specific for Dopamine Receptor D2 long form. Dopamine D2 receptor is known to exist in the short (D2S) and the long (D2L) forms that are encoded by splice variants of a single gene and differ only by the presence of an additional 29 amino acid in the intracellular, cytoplasmic loop 3 (Dal Toso et al. 1989; Grandy et al. 1989; Monsma et al. 1990; Giros et al. 1989). D2L receptor is a 446 aa, G-protein coupled and transmembrane receptor protein (Boundy et al. 1993) The 27 aa rat D2RL peptide used for immunization was found only in the D2 long form and there is no significant homology with other dopamine receptors (D1, D2 short, and D3-D5).

SPECIES REACTIVITIES: The immunogen peptide shows sequence homology of 96% with human/bovine (26/27 aa), 62% with Xenopus (17/27 aa) D2L receptors. Antibodies generated to the rat sequence have been shown to recognize the canine D2R (Boundy et al. 1993). Antibody cross-reactivity with various species is not known.

Immunogen

A 27 amino acid peptide sequence from the rat D2 long form within the cytoplasmic loop #3.

Epitope: cytoplasmic domain, long form

Application

Detect Dopamine D2 Receptor using this Anti-Dopamine D2 Receptor Antibody, cytoplasmic domain, long form validated for use in ELISA, IH, IP & WB.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Western blot: 1-10 μg/mL (Chemiluminescence technique) The predicted size of D2L form is approximately 50 kDa. However, additional bands at approximately 60 and 100 kDa, and others are typically seen in various tissues (Boundy, et al. 1993; Sakata, et al. 1992; Farooqui et al. 1991, 1992; Brock et al. 1992; Farooqui & Prasad 1992). Approximately 100-400 μg of membrane protein/lane should be loaded to visualize various bands.

Immunohistochemistry: 1-20 μg/mL on paraformaldehyde fixed sections.

Immunoprecipitation: 1-10 μg using 100-400 μg of protein (Boundy, et al. 1993).

ELISA: 0.5-1.0 μg/mL (1 μg/mL immunogen peptide (AG211)/well)

Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified immunoglobulin. Liquid at 1.0 mg/mL in PBS, pH 7.4 with 0.1% BSA and 0.02% sodium merthiolate.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: canine, rat. manufacturer/tradename: Chemicon^®. . technique(s): ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NM_000795.2, NM_016574.2. UniProt accession no.: P14416. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: dog ... Drd2(403701)
rat ... Drd2(24318). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.