Specificity
Specific for Dopamine Receptor D3. Human Dopamine D3 receptor is a 400 aa, G-protein coupled and transmembrane receptor protein (Giros et al. 1990, 1991; Schmauss et al. 1993; Liu et al. 1994; Fu et al. 1995; Sokoloff et al. 1990; Livingstone et al. 1992). The immunogen peptide shows no significant sequence similarity with other dopamine receptors (D1, D2, D4 or D5).
Immunogen
A 19 amino acid peptide sequence from the human D3 receptor within the 3rd cytoplasmic domain.
Epitope: cytoplasmic domain
Application
Detect Dopamine D3 Receptor using this Anti-Dopamine D3 Receptor Antibody, cytoplasmic domain validated for use in ELISA, IH & WB.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Western blot: 1-10 μg/mL (Chemiluminescence technique) Approximately 100-400 μg of membrane protein/lane should be loaded to visualize the receptor band.
Immunohistochemistry: 1-20 μg/mL on paraformaldehyde fixed sections.
ELISA: 0.5-1.0 μg/mL (1 μg/mL immunogen peptide (Cat. Number AG224)/well)
Optimal working dilutions must be determined by the end user.
Physical form
Affinity purified immunoglobulin. Liquid in PBS, pH 7.5 with 0.1% BSA. No preservatives
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. manufacturer/tradename: Chemicon®. . technique(s): ELISA: suitable, immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NM_000796.3, NM_033663.3. UniProt accession no.: P35462. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... DRD3(1814). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51392403
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB1785P