Anti-Dopamine D3 Receptor Antibody, cytoplasmic domain (C15-1315-892)

Specificity

Specific for Dopamine Receptor D3. Rat Dopamine D3 receptor is a 446 aa, G-protein coupled and transmembrane receptor protein (Giros et al. 1990, 1991; Schmauss et al. 1993; Liu et al. 1994; Fu et al. 1995; Sokoloff et al. 1990; Livingstone et al. 1992). The immunogen peptide shows no significant homology with other dopamine receptors (D1, D2, D4 or D5).

SPECIES REACTIVITIES: The immunogen peptide shows sequence homology of 88% with mouse and 63% with human/monkey (12/19 aa) D3 receptors (Giros et al. 1990, 1991; Schmauss et al. 1993; Liu et al. 1994; Fu et al. 1995; Sokoloff et al. 1990; Livingstone et al. 1992). Antibody cross-reactivity with various species is not known.

Immunogen

A 19 amino acid peptide sequence from the rat D3 receptor within the 3rd cytoplasmic domain.

Epitope: cytoplasmic domain

Application

Anti-Dopamine D3 Receptor Antibody, cytoplasmic domain detects level of Dopamine D3 Receptor & has been published & validated for use in ELISA, IH & WB.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Western blot: 1-10 μg/mL (Chemiluminescence technique; 45-50 kDa) Approximately 100-400 μg of membrane protein/lane should be loaded to visualize the receptor band. In some systems 70kDa bands may also be present.

Immunohistochemistry: suggested working concentration is 2-10 μg/mL. Suggested fixative is 4% PFA, fixation 10-15′, permeabilized with 0.3-0.5% triton X-100 for 10-15′ (tissue, less time for cels), primary antibody dilution is 1:100 for direct fluorescence . {Coronas, V, et al. J Neurochemistry 91:1292-1301, 2004},

ELISA: 0.5-1.0 μg/mL (1 μg/mL immunogen peptide (Catalog Number AG229)/well)

Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse, rat. manufacturer/tradename: Chemicon^®. . technique(s): ELISA: suitable, immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NM_000796.3, NM_033663.3. UniProt accession no.: P35462. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: mouse ... Drd3(13490)
rat ... Drd3(29238). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.