ANTI-DOT1L CLONE 1A11.2

General description

Histone-lysine N-methyltransferase, H3 lysine-79 specific (EC 2.1.1.43; UniProt Q8TEK3; also known as Disruptor of telomeric silencing-1 like, DOT1-like protein, H3-K79-HMTase, Histone H3-K79 methyltransferase, Lysine N-methyltransferase 4) is encoded by the DOT1L (also known as KIAA1814, KMT4) gene (Gene ID 84444) in human. Disruptor of telomeric silencing-1 like (DOT1L) catalyzes the mono-, di-, and tri-methylation of histone H3 on lysine-79 (H3K79). DOT1L-mediated H3K79 methylation is associated with active transcription. Accumulating evidences indicate a role of DOT1L and H3K79 in many biological processes, including DNA damage response, cell cycle, embryonic cell development, as well as the initiation and maintenance of mixed lineage leukemia (MLL)-rearranged leukemia. MLL fusion proteins such as AF9, AF10, and ENL recruit DOT1L to their target gene loci to help promote the transcription of genes that contribute to leukemic transformation of hematopoietic progenitor cells. DOT1L also has been identified as a coactivator of Wnt signaling in colorectal cancer, and is shown to induce neoplastic transformation of immortalized human breast epithelial cells and increase tumor initiation and growth of human breast cancer cells. In addtion, DOT1L is reported to regulate androgen receptor activity via direct methylation.

Specificity

Clone 1A11.2 detects both spliced isoforms of human DOT1L reported by UniProt (Q8TEK3).

Immunogen

Epitope: Internal (N-terminal half).

KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human DOT1L.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histone Modifying Proteins

This Anti-DOT1L Antibody, clone 1A11.2 is validated for use in Western Blotting for the detection of DOT1L.

Quality

Evaluated by Western Blotting in Jurkat nuclear extract.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected DOT1L isoforms in 10 µg of Jurkat nuclear extract.

Target description

~185/165 kDa observed. 164.9 kDa (isoform 1; Q8TEK3-2) and 184.9 kDa (isoform 2; Q8TEK3-1) calculated.

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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