General description
Anti-Doublecortin, Cat. No. AB2253, is a guinea pig polyclonal antibody that detects Doublecortin and is tested for use in Immunohistochemistry (Paraffin) and Immunofluorescence, and Western Blotting.
Specificity
Reactivity with other species has not been determined.
This Guinea pig polyclonal antibody detects doublecortin in murine and rat species. It targets an epitope with in 17 amino acids from the C-terminal region.
Immunogen
Epitope: C-Terminus
Recombinant Peptide.
Application
Anti-Doublecortin Antibody has been used in immunohistochemistry and immunofluorescence staining.
Quality
Evaluated by Western Blotting in rat brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Doublecortin in rat brain tissue lysate.
Target description
~45 kDa observed; 40.57 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Linkage
Replaces: AB5910
Physical form
GST Depletion
GST-Depleted Guinea Pig Sera with 0.05% Sodium Azide
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Rat Brain lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: guinea pig. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. mol wt: (Target molecular weight ~42 kDa observed. 40.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s).). species reactivity: rat, mouse. species reactivity (predicted by homology): human (based on 100% sequence homology). technique(s): immunofluorescence: suitable (Frozen tissue sections), immunohistochemistry: suitable (Paraffin), western blot: suitable. NCBI accession no.: NC_000086.7. UniProt accession no.: O88809. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... DCDC1(341019). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51201516
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB2253