Anti-DR5 Antibody, CT (C15-1315-867)

Specificity

Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family. DR4 was recently identified as the receptor for TRAIL. A novel death domain containing receptor for TRAIL was more recently identified and designated DR5, Apo2, TRAIL-R2, TRICK2 or KILLER by several groups independently (Pan et al. 1997; Sheridan et al. 1997; Walczak et al. 1997; MacFarlane et al. 1997; Screaton et al. 1997; Wu et al. 1997; Chaudhary et al. 1997). Like DR4, DR5 transcript is widely expressed in normal tissues and in many types of tumor cells. DR5 binds to TRAIL and mediates TRAIL induced cell death. Overexpression of DR5 induces apoptosis and activates NF-kB.

Immunogen

Epitope: C-terminus

Polyclonal antibody raised against a peptide corresponding to amino acids 388-407 of human DR5 precursor (Pan et al. 1997; Sheridan et al. 1997).

Application

Anti-DR5 Antibody, C-terminus detects level of DR5 & has been published & validated for use in WB.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western blot: 1:250 to 1:500

Whole cell lysate from HeLa or K562 cells can be used as a positive control .

Optimal working dilutions must be determined by end user.

Physical form

Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.

Format: Purified

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Other Notes

Click here for more information on observed vs. calculated molecular weight.


Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. mol wt: calculated mol wt-47.88 . kDa, observed mol wt ~55 . kDa. species reactivity: human. manufacturer/tradename: Chemicon^®. . technique(s): western blot: suitable. NCBI accession no.: NM_003842.3, NM_147187.1. UniProt accession no.: O14763. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... TNFRSF10B(8795). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.