ANTI-DUSP6 CLONE 1H14 ZOOMAB« RABBIT MONOCLONAL (C15-1669-489)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1H14 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Dual specificity protein phosphatase 6 (DUSP6). It targets an epitope within 24 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal region of human Dual specificity protein phosphatase 6 (DUSP6).

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected DUSP6 in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected DUSP6 in rat liver tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100-1,000 dilution from a representative lot detected DUSP6 in human liver and human cerebral cortex tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected DUSP6 in HepG2 cells.

Affinity Binding Assay: A representative lot of this antibody bound DUSP6 peptide with a KD of 1.3 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Dual specificity protein phosphatase 6(UniProt: Q16828, also known as EC:3.1.3.16, Dual specificity protein phosphatase PYST1, Mitogen-activated protein kinase phosphatase 3, MAP kinase phosphatase 3. MKP-3) is encoded by the DUSP6 (also known as MKP3, PYST1) gene (Gene ID: 1848) in human. DUSP6 is a member of the dual-specificity phosphatases family that serves as an ETS-regulated negative modulator of ERK pathway. It mediates the dephosphorylation of MAPKs on threonine/serine and tyrosine residues. Its tyrosine phosphatase domain is localized in amino acids 206-349. It is essential for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia. Dephosphorylation of MAPK1/3 in local wound tissue contributes to resolution of acute pain. It is reported to promote cell differentiation by regulating MAPK1/MAPK3 activity and regulating the expression of AP1 transcription factors. It plays an important role in numerous types of solid tumors as a tumor suppressor and its levels are down-regulated in several types of cancers, including pancreatic, lung, ovarian cancers, and esophageal squamous cell carcinoma. Loss of DUSP6 results in hyperactivation of ERK1/2 that leads to higher cell proliferation, tumorigenicity, and chemoresistance. Two isoforms of DUSP6 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Skopelja-Gardner, S., et al. (2017). J. Pain Res.10; 763-774; Ma, J., et al. (2013). Oncol. Lett. 6(6); 1624-1630; Zhnag, Z., et al. (2010). Carcinogenesis. 31(4); 577-586).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.