General description
Dux4 is a homeodomain protein with a similar protein sequence to Pax3 and Pax7. Defects in Dux4 may be the cause of facioscapulohumeral muscular dystrophy (FSHD). FSHD is characterized by weakness of the muscles of the face, upper-arm and shoulder girdle. Ectopic expression of Dux4 in skeletal muscle activates the expression of stem cell and germline genes, and, when overexpressed in somatic cells, Dux4 can ultimately lead to cell death.
Immunogen
Epitope: domain of: C-Terminal region
recombinant protein corresponding to the domain of: C-Terminal region of Human Dux4.
Application
Detect Dux4 using this mouse monoclonal antibody, Anti-Dux4 Antibody, clone 9A12 validated for use in western blotting, ICC, Electrophoretic Mobility Shift Assay & IP.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Muscle Physiology
Developmental Signaling
This antibody has been shown to work in immunocytochemistry applications (Dixit et al. 2007). This antibody has been shown to work in electrophoretic mobility shift assays (Dixit et al. 2007). This antibody has been shown to work in immunoprecipitation applications (Anseau et al., 2009).
Quality
Evaluated by Western Blot in a human skeletal muscle tissue lysate. Western Blot Analysis: A 1:250 dilution of this antibody detected Dux4 in a human skeletal muscle tissue lysate.
Target description
~58 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human skeletal muscle tissue lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Shipping Information:
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- UPC:
- 51202407
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MABD116
- Temperature Control Device:
- Yes
