Anti-DYKDDDDK (FLAG) Tag (FG4R) Antibody (Mouse Monoclonal IgG), 100 g

Anti-DYKDDDDK (FLAG) Tag (FG4R) mAb recognizes N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins. Description: Anti-DYKDDDDK (FLAG) Tag (FG4R) Monoclonal Antibody Clonality: FG4R Host Species: Mouse Isotype: IgG2b Immunogen: DYKDDDDK (FLAG) synthetic peptide conjugated to KLH Specificity: Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins Purification: Protein A affinity chromatography from mouse ascites fluid Applications: Dot, ELISA, IP, IS, WB (see notations) Sensitivity: WB (with ECL): 1-5ng of DYKDDDDK-tagged fusion proteins Form: 1mg/mL in 10mM PBS, pH 7.2 Working Conditions: WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test Storage: Store at 4°C (add 0.1% NaN3) for several days to weeks, and at -20°C for longer periods. Avoid repeated freeze-thaw cycles. Product Type : Tag Abs / Tag Antibodies / Tag Antibody