Anti-Dysbindin (C15-1317-405)

General description

Dysbindin (UniProt: Q96EV8; also known as Biogenesis of lysosome-related organelles complex 1 subunit 8, BLOC-1 subunit 8, Dysbindin-1, Dystrobrevin-binding protein 1, Hermansky-Pudlak syndrome 7 protein, HPS7 protein) is encoded by the DTNBP1 (also known as BLOC1S8, My031) gene (Gene ID: 84062) in human. Dysbindin is a component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. It plays a role in synaptic vesicle trafficking and in neurotransmitter release. Three isoforms produced by alternative splicing and alternative initiation have been described. Isoform 1 is mainly cytoplasmic, but shuttles between the cytoplasm and nucleus. It is exported out of the nucleus via its nuclear export sequence (NES). Its nuclear localization is required for regulation of the expression of genes, such as SYN1. It is detected in neuron cell bodies, axons and dendrites and is mainly located to the postsynaptic density. Isoform 2 also shuttles between the cytoplasm and nucleus and is mainly expressed in the dendritic spine. It is predominantly a synaptic vesicle isoform but also highly expressed in the nucleus. Isoform 3 is exclusively cytoplasmic and is predominantly found in the postsynaptic density (PSD) with little association with synaptic vesicles. Mutations in DTNBP1 gene have been linked to Hermansky-Pudlak syndrome 7 (HPS7) that is characterized by oculocutaneous albinism, bleeding due to platelet storage pool deficiency, and lysosomal storage defects. Defects in DTNBP1 are associated with susceptibility to schizophrenia.

Specificity

This polyclonal antibody detects Dysbindin in various regions of human, mouse, and rat brain.

Immunogen

MBP-tagged full length recombinant human Dysbindin.

Application

Anti-Dysbindin, Cat. No. ABN1653, is a highly specific rabbit polyclonal antibody that targets Dysbindin-1 and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunofluorescence, Immunoprecipitation, and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Dysbindin in human cerebral cortex, human colon, and human kidney tissue.

Immunohistochemistry Analysis: A representative lot detected Dysbindin in Immunohistochemistry applications (Ito, H., et. al. (2010). Mol Psychiatry. 15(10):976-86).

Immunofluorescence Analysis: A 1,000 dilution from a representative lot detected Dysbindin in P8 mouse brain hippocampus tissue (Courtesy of Dr Koh-ichi Nagata at Aichi Human Service Center).

Immunoprecipitation Analysis: A representative lot detected Dysbindin in Immunoprecipitation applications (Ito, H., et. al. (2010). Mol Psychiatry. 15(10):976-86).

Immunocytochemistry Analysis: A representative lot detected Dysbindin in Immunocytochemistry applications (Yuan, Q., et. al. (2016). Biol Psychiatry. 80(4):312-22).

Western Blotting Analysis: A representative lot detected Dysbindin in Western Blotting applications (Lee, S.A., et. al. (2015). J Biol Chem. 290(11):7087-96).

Immunoprecipitation Analysis: A representative lot detected Dysbindin in Immunoprecipitation applications (Lee, S.A., et. al. (2015). J Biol Chem. 290(11):7087-96).

Western Blotting Analysis: A representative lot detected Dysbindin in Western Blotting applications (Ito, H., et. al. (2010). Mol Psychiatry. 15(10):976-86).

Immunocytochemistry Analysis: A representative lot detected Dysbindin in Immunocytochemistry applications (Ito, H., et. al. (2010). Mol Psychiatry. 15(10):976-86).

Research Category
Neuroscience

Quality

Evaluated by Immunohistochemistry in human cerebellum tissue.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Dysbindin in human cerebellum tissue.

Target description

39.49 kDa calculated.

Linkage

Replaces: AB15450

Physical form

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 100 mM sodium phosphate buffer (pH 7.0), 20 mg/ml BSA with 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, mouse, rat. technique(s): immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_115498.2. UniProt accession no.: Q96EV8. shipped in: ambient. target post-translational modification: unmodified. Gene Information: human ... DTNBP1(84062). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.