General description
Anti-EDN3, mouse monoclonal, clone 2A6-2A4, recognizes the ~26 kDa EDN3 protein in LNCaP cells. it is validated for use in ELISA and Western blotting.
Purified mouse monoclonal antibody. Recognizes the ~26 kDa EDN3 protein.
Recognizes the ~26 kDa EDN3 protein in LNCaP cells.
Immunogen
Full-length, recombinant human EDN3 (aa 1-239), expressed as a GST fusion protein
Application
ELISA (see comments)
Immunoblotting (1-5 g/ml)
Warning
Toxicity: Regulatory Review (Z)
Physical form
In PBS, pH 7.2.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
293T cells
Positive Control
LNCaP cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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- UPC:
- 51204100
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- ST1513-100UG
- Temperature Control Device:
- Yes