Anti-Ena/VASP-like protein Antibody (C15-1318-075)

General description

Ena (VASP-like protein) is involved in many different cellular functions including cell migration and axon guidance. Studies have shown that Ena expression is significantly increased in breast cancer and that the level of expression is associated with tumor stage. Ena has also been linked to inner ear and hair cell development.

Specificity

This antibody recognizes Ena/VASP-like protein at the EVH2 region.

Immunogen

Epitope: EVH2 region

KLH-conjugated linear peptide corresponding to the EVH2 region of human Ena/VASP-like protein.

Application

Detect Ena/VASP-like protein using this Anti-Ena/VASP-like protein Antibody validated for use in WB & IC.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Ena/VASP-like protein in NIH/3T3 cells.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Quality

Evaluated by Western Blot in Ramos cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Ena/VASP-like protein in 10 µg of Ramos cell lysate.

Target description

~46 kDa observed.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Ramos cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, mouse. species reactivity (predicted by homology): rat (based on 100% sequence homology), chimpanzee (based on 100% sequence homology). technique(s): immunocytochemistry: suitable, western blot: suitable. NCBI accession no.: NP_057421. UniProt accession no.: Q9UI08. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... EVL(51466). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.