ANTI-EPCAM CLONE 1B8 ZOOMAB RABBIT MONOCLONAL (C15-1670-926)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

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Specificity

Clone 1B8 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Epithelial cell adhesion molecule (EpCAM). It targets an epitope within the extracellular domain.

Immunogen

GST/His-tagged recombinant fragment corresponding to 140 amino acids from the extracellular domain of human Epithelial cell adhesion molecule (EpCAM).

Application

Anti-EpCAM, clone 1B8 ZooMAb, Cat. No. ZRB1215, is a highly specific rabbit recombinant monoclonal antibody that targets Epithelial cell adhesion molecule (EpCAM) and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:100-1,000 dilution from a representative lot detected EpCAM in HT-29 and MCF7 cell lysates.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected EpCAM in human small intestine tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:10 dilution from a representative lot detected EpCAM in human colon cancer and human cervical cancer tissue sections.

Flow Cytometry Analysis: 1 µg of a representative lot of this antibody detected EpCAM in one million fixed and permeabilized HT29 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary.

Target description

Epithelial cell adhesion molecule (UniProt: P16422; also known as Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, EGP, Epithelial glycoprotein 314, EGP314, hEGP314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, CD326) is encoded by the EPCAM (also known as GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1) gene (Gene ID: 4072) in human. Ep-CAM is single-pass type I membrane monomeric protein that may act as a physical homophilic interaction molecule between intestinal epithelial cells and intraepithelial lymphocytes at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. It plays a role in embryonic stem cells proliferation and differentiation. It is selectively expressed by undifferentiated cells and its levels decline rapidly upon embryonic stem cell differentiation. Its expression is observed in almost all epithelial cell membranes, but not on mesodermal or neural cell membranes. It is also reported to be expressed on the surface of adenocarcinoma. On carcinoma tissue it is found in a hyperglycosylated state. Glycosylation at asparagine 198 is shown to be essential for its stability. EpCAM is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 24-265), a short transmembrane domain (aa 266-288), and the cytoplasmic domain (aa 289-314). Mutations in EPCAM gene have been linked to Hereditary non-polyposis colorectal cancer 8 that is characterized by a familial predisposition to early-onset colorectal carcinoma and extra-colonic tumors of the gastrointestinal, urological and female reproductive tracts. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.