Anti-Equilibrative Nucleoside Transporter 1 Antibody (C15-1317-581)

General description

Equilibrative Nucleoside Transporter 1 (ENT1), also known as Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter, Equilibrative NBMPR-sensitive nucleoside transporter, Nucleoside transporter, es-type, or Solute carrier family 29 member 1, and encoded by the gene SLC29A1/ENT1, is a transport membrane protein that is responsible for mediating the transport of nucleoside molecules in and out of cells. Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. ENT1 is spatially localized to the basolateral cell membranes and is found expressed in many organs including heart, brain, mammary glands, liver and spleen. It is up regulated in cancer cells but it is also an anti-cancer target as it is sensitive to the chemotherapeutic drugs dipyridamole and dilazep.

Immunogen

Epitope: cytoplasmic domain

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Equilibrative Nucleoside Transporter 1. Immunogen is 80% identical to mouse and rat ENT1.

Application

Immunohistochemistry Analysis: A 1:500-2,000 dilution from a representative lot detected Equilibrative Nucleoside Transporter 1 in human cerebellum, human breast cancer, and human cerebral cortex tissue.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

This Anti-Equilibrative Nucleoside Transporter 1 antibody is validated for use in western blotting & IHC (Paraffin) for the detection of Equilibrative Nucleoside Transporter 1.

Quality

Evaluated by Western Blotting in human heart tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Equilibrative Nucleoside Transporter 1 in 10 µg of human heart tissue lysate.

Target description

~50 kDa observed

Linkage

Replaces: AB15267

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. species reactivity (predicted by homology): bovine (based on 100% sequence homology), nonhuman primates (based on 100% sequence homology). technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable. NCBI accession no.: NP_004946. UniProt accession no.: Q99808. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... SLC29A1(2030). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.