Anti-ERR-alpha P2 Antibody (C15-1317-216)

General description

ERR alpha is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site specific transcription regulator and has also been shown to interact with estrogen and the transcription factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium chain acyl coenzyme A dehydrogenase (MCAD),and thyroid hormone receptor genes. ERR alpha is expressed in many adult and embryonic tissues (particularly at the onset of ossification) as well as in several osteoblast cell lines. ERR alpha expression has been documented in brain, spinal cord, pituitary gland and heart. More recently, ERR alpha has been observed in various human breast cancer cell lines, breast adipose tissue and breast tumors. Studies demonstrating the use of ERR alpha antagonists in treating breast cancer have excited great interest in this receptor in the search for curing the disease (Chisamore, 2009).

Specificity

This antibody recognizes ERR-alpha.

Immunogen

KLH-conjugated linear peptide corresponding to human ERR-alpha.

Application

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected ERR-alpha in A431 and HeLa cells.

Use Anti-ERR-alpha P2 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect ERR-alpha P2 also known as Estrogen receptor-like 1, Estrogen-related receptor alpha.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected ERR-alpha on 10 µg of HeLa cell lysate.

Target description

~ 53 kDa

Linkage

Replaces: 04-1134

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

biological source: rabbit. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human, mouse. technique(s): immunocytochemistry: suitable, western blot: suitable. isotype: IgG. NCBI accession no.: NP_004442. UniProt accession no.: P11474. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... ESR1(2099). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.