ANTI-EWS CLONE 21B1 (C15-1887-703)

General description

Heterogeneous nuclear ribonucleoprotein D0 (UniProt: Q14103; also known as hnRNP D0, AU-rich element RNA-binding protein 1, AUF1) is encoded by the HNRNPD (also known as AUF1, HNRPD) gene (Gene ID: 3184) in human. AUF1 is one of several proteins that exhibits high-affinity binding and specificity for AU-rich elements (AREs) in 3 untranslated regions (3 UTRs). It also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. AUF1 is also considered as an RNA destabilizing protein. Four isoforms of AUF1 have been described that are produced by alternative splicing. Although the role of each isoform has not been fully characterized, a direct correlation has been observed between each AUF1 isoform s binding affinity and its RNA destabilizing activity toward different AREs, with the isoforms p37 and p42 being the most effective. It contains several RNA binding domains and each of these specifically can bind solely to a single-stranded non-monotonous 5′-UUAG-3′ sequence and also to the single-stranded 5′-TTAGGG-3′ telomeric DNA repeat in a weaker manner. AUF1 is reported to be localized in cytoplasmic mRNP granules containing untranslated mRNAs and its cytoplasmic localization oscillates diurnally. AUF1 is also shown to a bcl2 mRNA binding protein and all four isoforms can form complexes with bcl2 ARE. AUF1 is also involved in the downregulation of ARE mediated bcl2 mRNA during apoptosis. Clone 5B9 is shown to bind to an epitope within the RNA binding domain II (RBD2). This is a common epitope present in RBD2 of all isoforms of AUF1. (Ref.: Dogar, MA., et al. (2018). PLoS One. 13(11); e0206823; Kajita, Y., et al. (1995). J. Biol. Chem. 270(38); 22167-22175; Ishikawa, F., et al. (1993). Mol. Cell Biol. 13(7); 4301-4310).

Specificity

Clone 5B9 is a mouse monoclonal antibody that detects human AU-rich element RNA-binding protein 1 (AUF1). It targets an epitope within the RBD2 domain from the internal region.

Immunogen

Human AUF1

Application

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in human stomach tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected AUF1 in human stomach tissue sections.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected AUF1 in human testis tissue sections.

Western Blotting Analysis: A representative lot detected AUF1 in Western Blotting applications (Gouble, A., et al. (2002). Cancer Res. 62(5):1489-95).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected AUF1 in Human stomach tissue lysates.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-AUF1, clone 5B9, Cat. No. MABE1991, is a mouse monoclonal antibody that detects AU-rich element RNA-binding protein 1 (AUF1) and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.