ANTI-EWS CLONE 21B1 (C15-1887-717)

General description

Pescadillo homolog (UniProt: O00541; also known as PES1) is encoded by the PES1 gene (Gene ID: 23481) in human. PES1 is a component of the evolutionary conserved stable PeBOW-complex that is composed of Pes1 (Pescadillo), Bop1 (Bop1 (block of proliferation) and WDR12 (WD-repeat protein) in mammalian cells. This complex is essential for cell proliferation. It is required for maturation of the 28S and 5.8S ribosomal RNAs and for the formation of 60S ribosome. PES1 is localized in the nucleolus and localizes to the periphery of metaphase chromosomes during mitosis and to the prenucleolar bodies that form in mitotic cells prior the actual nucleoli. Transient deletion of PES1 is reported to result in abnormal mitosis with binucleate or hyperploid cells with multipolar spindles. PES1 contains a BRCT domain (aa 322-415) and either deletion or single point mutations in highly conserved residues in this domain can cause diffuse nucleoplasmic distribution and failure to replace endogenous PES1 in rRNA processing. Point mutations in BRCT-domain can negatively affect PES1 stability and prevent its incorporation into the PeBoW-complex. Higher levels of PES1 are observed in a variety of cancer cell lines, including glioblastoma, breast carcinoma, colon carcinoma, and in malignant tumors of astrocyte origin. Two isoforms of human PES1 have been described that are produced by alternative splicing. (Ref.: Holzel, M., et al. (2007). Nucleic Acids Res. 35(3); 789-800; Grimm, T., et al. (2006). Nucleic Acids Res. 34(10); 3030-3043; Holzel, M., et al. (2005). J. Cell Biol. 170 (3); 367-378).

Specificity

Clone 8E9 is a rat monoclonal antibody that detects Pescadillo Homolog (PES1). It targets an epitope within 22 amino acids from the C-terminal region.

Immunogen

Ovalbumin-conjugated linear peptide corresponding to 22 amino acids from the C-terminal region of human Pescadillo Homolog (PES1).

Application

Quality Control Testing

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PES1 in U2OS cell lysate.

Tested Applications

Western Blotting Analysis: A representative lot detected PES1 in Western Blotting applications (Hölzel, M., et al. (2005). J Cell Biol. 170(3):367-78; Grimm, T., et al. (2006). Nucleic Acids Res. 34(10):3030-43; Hölzel, M., et al. (2007). Nucleic Acids Res. 35(3):789-800; Burger, K., et al. (2010). J Biol Chem. 285(16):12416-25; Hölzel, M., et al. (2010). J Biol Chem. 285(9):6364-70).

Immunoprecipitation Analysis: A representative lot detected PES1 in Immunoprecipitation applications ( Hölzel, M., et al. (2005). J Cell Biol. 170(3):367-78).

Immunofluorescence Analysis: A representative lot detected PES1 in Immunofluorescence applications (Burger, K., et al. (2010). J Biol Chem. 285(16):12416-25).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-PES1, clone 8E9, Cat. No. MABE1945, is a rat monoclonal antibody that detects Pescadillo homolog (PES1) and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

Physical form

Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.