ANTI-EWS CLONE 21B1 (C15-1887-720)

General description

Transcription factor SOX-11 (UniProt: P35716; also known as SOX-11) is encoded by the SOX11 gene (Gene ID: 6664) in human. Sox-11 is a neural transcription factor that is involved in the embryonic neurogenesis and plays a role in tissue modeling during development, neuronal cell proliferation, and survival. Its expression is primarily observed in the brain and heart and low expression is detected in the kidney, pancreas, and muscle. Its transient activity during neurogenesis is critical to ensure the precise execution of the neurogenic program. Sox-11 serves as a transcription factor and acts as a transcriptional activator. It binds cooperatively with Brn2 or Oct6, which enhances its transcriptional activation. Sox-11 activity is controlled by epigenetics and transcriptional mechanisms and by post-translational modifications. Its three highly conserved phosphorylation sites, serine 30, 133, and 137, surrounding the DNA-binding high-mobility group (HMG box), are reported to modulate its transcriptional activity. These sites are unique to Sox-11. Serine 30 phosphorylation is reported to promote its nuclear over cytoplasmic localization. Phosphorylation of serine 133 by PKA modulates dendrite development of adult-born dentate granule neurons and fine tunes the activated of selected target genes. Higher expression of Sox-11 has been shown in Mantle cell lymphoma. Mutations in SOX11 gene are known to cause Coffin-Siris syndrome 9 that is characterized multiple malformations and intellectual disability. Mice deficient in Sox-11 exhibit defects in multiple organs including the lung, heart, and pancreas.

Specificity

Clone SOX11-C1 is a mouse monoclonal antibody that detects human Sox-11. It targets an epitope within the internal region.

Immunogen

His-tagged recombinant fragment corresponding to 121 amino acids from the internal region of human Sox-11.

Application

Quality Control Testing

Evaluated by Immunocytochemistry in SH-SY5Y cells.

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected Sox-11 in SH-SY5Y cells.

Tested Applications

Flow Cytometry Analysis: A representative lot detected Sox-11 in Flow Cytometry applications (Nordstrom, L., et al. (2012). BMC Cancer.;12:269; Lu, T.X., et al. (2013). Leuk. Res. 37(11):1412-9).

Western Blotting Analysis: A representative lot detected Sox-11 in Western Blotting applications (Nordstrom, L., et al. (2012). BMC Cancer. 12:269).

Immunocytochemistry Analysis: A representative lot detected Sox-11 in Immunocytochemistry applications (Nordstrom, L., et al. (2012). BMC Cancer. 12:269).

Immunohistochemistry (Paraffin) Analysis: A representative lot detected Sox-11 in Immunohistochemistry applications (Nordstrom, L., et al. (2012). BMC Cancer. 12:269; Nordstrom, L., et al. (2014). Br. J. Haematol. 166(1):98-108; Lu, T.X., et al. (2013). Leuk Res. 37(11):1412-9).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Sox-11, clone SOX11-C1, Cat. No. MABE1935, is a mouse monoclonal antibody that detects SOX-11 and is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.