ANTI-EZRIN CLONE 1D15 ZOOMAB RB MONO (C005B-262101)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1D15 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Ezrin. It targets an epitope with 15 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Ezrin.

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ezrin in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Ezrin in MCF-7, NIH3T3, and C6 cell lysates.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Ezrin in A431 and HepG2 cells.

Flow Cytometry Analysis: 1 μg from a representative lot detected Ezrin in one million HeLa cells.

Affinity Binding Assay: A representative lot of this antibody bound Ezrin peptide with a KD of 9.5 x 10-7 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Ezrin in human kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Ezrin, clone 1D15 ZooMAb^®, Cat. No. ZRB1253, is a recombinant Rabbit monoclonal antibody that specifically targets Ezrin and has been tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and WB.

Target description

Ezrin (UniProt: P15311; also known as Cytovillin, Villin-2, p81) is encoded by the EZR (also known as VIL2) gene (Gene ID: 7430) in human. Ezrin, a peripheral membrane protein, is a member of the ERM (Ezrin Radixin Moesin) family that function as cross-linkers between the actin cytoskeleton and the plasma membrane. ERM proteins are shown to be involved in microvilli formation and breakdown and are essential for cell-cell and cell-substrate adhesions. ERM proteins are involved in Rho GTPase signaling and are associated with cell morphology, migration, and growth. Ezrin expression has been reported in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Its stronger expression is detected in gray matter of frontal lobe compared to white matter. It is preferentially expressed in astrocytes of hippocampus, frontal cortex, and thalamus. It is also a component of the microvilli of intestinal epithelial cells where it localized to their apical surface. A head-to-tail association of the N-terminal and C-terminal halves of Ezrin result in a closed conformation (inactive form), which is incapable of actin or membrane-binding. Its phosphorylation by ROCK2 is reported to suppress this head-to-tail association resulting in an opened conformation that is capable of actin and membrane-binding. Overexpression of Ezrin has been associated with higher grade tumors and is associated with poor outcome in several cancers. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Gautreau, A., et al. (1999). Proc. Natl. Acad. Sci. USA 96(13); 7300-7305).

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.