ANTI-FANCA ANTIBODY CLONE 5G9

General description

FANCA, also named as FAA, FACA and FANCH, belongs to the multisubunit FA complex which is not found in patients with Fanconi anemia. FANCA may be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Mutations in FANCA have been associated with Fanconi anemia (FA) which is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of human FANCA.

Application

Anti-FANCA Antibody, clone 5G9 is a highly specific mouse monoclonal antibody, that targets FANCA & has been tested in western blotting & IP.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected FANCA in 10 µg of HeLa cell lysate.

Western Blotting Analysis: A representative lot from an independent laboratory detected FANCA in normal HSC93 and FA mutant lymphoblast whole cell extracts. The same lot also detected FANCA in HeLa whole cell extracts and in FANCA transfected PD430 whole cell extracts (Tremblay, C. S., et al. (2008). Blood. 112(5):2062-2070.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated FANCA from yeast extracts expressing FANCA. The same lot also immunoprecipiated FANCA from 293T whole cell extracts expressing FANCA (Tremblay, C. S., et al. (2008). Blood. 112(5):2062-2070.)

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated FANCA from FANCA wild type FA lymphoblast cell lysate (de Winter, J. P., et al. (2000). Hum Mol Genet. 9(18):2665-2674.).

Quality

Evaluated by Western Blotting in Raji cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FANCA in 10 µg of Raji cell lysate.

Target description

~165 kDa observed

Physical form

Format: Purified

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