General description
Fibroblast growth factor 2 (FGF-2) belongs to the family of heparin-binding growth factors. It is localized in the cells of the mesoderm and neuroectodermal origin and several tumor cells. FGF-2 exists in four isoforms. It is made up of four cysteine residues, many basic residues, and two active sites. FGF-2 protein is a trigonal pyramidal structure formed by twelve anti-parallel β-sheets. The FGF-2 chromosome is located on the human chromosome at 4q28.1.
Research area: Cancer
Specificity
This antibody is highly specific for the non-denatured form of FGF-2/basic FGF from bovine, human, rat and mouse sources. Does not cross-react with bovine acidic FGF.
This antibody recognizes the non-denatured form of FGF-2/basic FGF; does not cross-react with bovine acidic FGF.
Immunogen
Epitope: a.a. 5-24
Purified bovine brain basic FGF (HBGF-2). Clone bFM-1.
Application
Anti-FGF-2/basic FGF (neutralizing) Antibody, clone bFM-1 has been used in:
- FGF2 neutralization assay (6 or 12 µg/mL)
- in vitro tube formation assay (1000 ng/mL)
- immunolabelling (1:200)
Biochem/physiol Actions
Fibroblast growth factor 2 (FGF-2) plays a role in vertebrate embryonic and fetal development. It acts as an inducer of angiogenesis and is a well-known mitogen (in vitro) for a variety of cells including fibroblasts and vascular endothelial cells. FGF-2 exerts its biological activity through high-affinity tyrosine kinase receptors and low-affinity heparan sulfate proteoglycans present on the cell surface. It exhibits autocrine effects on various cell functions involved in angiogenesis such as proliferation, integrin expression, migration, and proteinase production. FGF-2 participates in the growth, migration, differentiation, and survival of a variety of cells. The binding of FGF-2 with heparin protects FGF-2 from denaturation by heat and acid and proteolytic cleavage.
Quality
Routinely evaluated by neutralization of greater than 50% of growth of FBHE cells simulated with FGF-2/bFGF.
Neutralization of bFGF Activity Assay:
2.5 µg/mL of this lot of antibody neutralized greater than 40% of growth of FBHE cells simulated with 5 ng/mL of FGF-2/bFGF.
Target description
17.5 kDa
Physical form
Ammonium sulfate precipitation and DEAE-cellulose chromatography
Format: Purified
Purified mouse monoclonal IgG1κ in TBS. Protein determined by a Bradford microtiter assay using mouse IgG as the standard. Frozen solution.
Storage and Stability
Stable for 1 year at -20ºC from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of the product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Fetal liver or kidney tissue.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Shipping Information:
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- UPC:
- 51201516
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 05-117
- Temperature Control Device:
- Yes
