Anti-Fibroblast Growth Factor Receptor-2, Extracellular antibody produced in rabbit

General description

Fibroblast growth factors (FGFs) belongs to the large tyrosine kinase family of growth factor receptors (17-38kDa). The FGF family consists of at least seventeen members designated FGF-1 through FGF-17 that bind to high affinity FGF receptors (FGFRs). Soluble, secreted or possibly cleaved forms of FGFR-1 and FGFR-2 have also been found in body fluids. They are glycosylated 110-150 kDa proteins that are constructed of an extracellular ligand binding region with either two, or typically three, immunoglobulin (Ig)-like domains and an eight amino acid "acidic box", a transmembrane region and a cytoplasmic split tyrosine kinase domain that is activated following ligand binding and receptor dimerization. The ligand binding site of all FGFRs is confined to the extracellular Ig-like domains 2 and 3.

Specificity

The antibody detects human FGFR-2 (bek) in extracts of transfected cells.

The antibody specifically reacts with human FGFR-2; no reaction with FGFR-1 or FGFR-3 is detected.

Immunogen

synthetic peptide corresponding to amino acids 362-374 of the extracellular region of human FGFR-2 (Bek).

Application

Anti-Fibroblast Growth Factor Receptor-2, Extracellular antibody produced in rabbit has been used in immunoprecipitation and immunoblotting.

Biochem/physiol Actions

Fibroblast growth factors (FGFs) are potent physiological regulators normal development, wound healing and repair, angiogenesis, a variety of neurotrophic activities, in hematopoiesis as well as in tissue remodeling and maintenance. FGFRs exhibit overlapping recognition and redundant specificity. Signal transduction by FGFRs requires dimerization or oligomerization and autophosphorylation of the receptors through their tyrosine kinase domain. Subsequent association with cytoplasmic signaling molecules leads to DNA synthesis or differentiation. The signaling and biological responses elicited by distinct FGFRs substantially differ. FGFR-2 is highly expressed in developing human tissues including the brain (preferentially in glial cells), choroid plexus, skin, lung, kidney and bone. It may be found in several anchorage-dependent cells, such as normal and malignant breast cancer cells.

Physical form

Solution in 0.01 M phosphate buffered saline,
pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Preparation Note

Affinity isolated on a peptide-agarose column.

Disclaimer

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