Anti-Fibronectin Antibody (C15-1315-944)

General description

Fibronectin is a high molecular weight extracellular adhesion molecule involved in many cellular processes, including tissue repair or wound healing, embryogenesis, blood clotting, and cell migration/adhesion. Fibronectin exists in two major forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the extracellular matrix (ECM), and 2) as a soluble disulfide-linked dimer found in the plasma. The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells. Fibronectin sometimes serves as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. It can also serve to organize cellular interaction with the ECM by binding to different components of the ECM and to membrane-bound fibronectin receptors on cell surfaces. The immunogen for fibronectin polyclonal antibody AB2033 is purified mouse plasma. The antibody shows less than 0.1% reactivity with mouse laminin, and collagen types I, III, and IV by radioimmunoassay.

Specificity

This antibody reacts with mouse fibronectin. Shows less than 0.1% reactivity with mouse Laminin, and Collagen Types I, III, and IV by radioimmunoassay.

Immunogen

Fibronectin extracted and purified from mouse plasma.

Application

Detect Fibronectin using this Anti-Fibronectin Antibody validated for use in ELISA, IC, IH, RIA.

Immunohistochemistry:
1:80 dilution of a previous lot was used in immunofluorescent staining of frozen mouse skin and liver tissues.

Radioimmunoassay:
A previous lot of this antibody was used in RIA.

ELISA:
A 1:200 dilution of a previous lot was used in ELISA.

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Quality

Evaluated by immunocytochemistry on NIH/3T3 cells.

Immunocytochemistry:
Representative image from a previous lot.
Confocal fluorescent analysis of NIH/3T3 using AB2033 rabbit polyclonal antibody (Red). Actin filaments have been labeled with AlexaFluor 488 -Phalloidin (Green). Nucleus is stained with DAPI (Blue).

Physical form

ImmunoAffinity Purified

The purified antibody is supplied in a buffer containing a mixture of 0.1M Citrate, 0.1M potassium phosphate, at a pH of 7.2-7.4 and 10µg/ml of antibiotics and antimytotics

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Extracellular matrix in most tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse. manufacturer/tradename: Chemicon^®. . technique(s): ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, radioimmunoassay: suitable. NCBI accession no.: NM_002026.2. UniProt accession no.: P02751. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: mouse ... Fn1(14268). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.