General description
Focal adhesion kinase family interacting protein of 200 kDa (FIP200) is a novel protein inhibitor for focal adhesion kinase (FAK). FIP200 inhibits kinase activity and associated cellular functions, such as cell adhesion, spreading, and motility in fibroblasts by binding directly to FAK.
The previously assigned protein identifier Q86YR4 has been merged into Q8TDY2. Full details can be found on the UniProt database.
Specificity
This antibody binds to the interaction domain of FIP200, which is needed for the formation of the ULK-FIP200 complex under starved, autophagy conditions.
Immunogen
Epitope: Interaction domain
GST-tagged recombinant protein corresponding to the interaction domain of human FIP200.
Application
Anti-FIP200 Antibody, clone 14E11.2 is an antibody against FIP200 for use in Western Blotting, ICC.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Western Blot Analysis: 1 µg/mL from a representative lot detected FIP200 in serum starved and non-serum starved HeLa cell lysate.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected FIP200 in NIH/3T3, A431, and HeLa cells.
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected FIP200 in untreated and chloroquinone-treated HUVEC cells.
Quality
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected FIP200 in 10 µg of HeLa cell lysate.
Target description
~200 kDa observed. An uncharacterized band may be observed at ~50 kDa in some cell lysates.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Shipping Information:
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- UPC:
- 51203902
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MABC128
- Temperature Control Device:
- Yes
